Syka and fies have described the theoretical advantagesof2dversus3dquadrupoleiontrapsforfourier transform mass spectrometry 14. Precursor ions are selected in the first mass analyzer and then subject to collisioninduced dissociation in the collision cell. Practical aspects of ion trap mass spectrometry, volume i 1st editio. A quadrupole ion trap is a type of ion trap that uses dynamic electric fields to trap charged particles. They are also called radio frequency rf traps or paul traps in honor of wolfgang paul, who invented the device and shared the nobel prize in physics in 1989 for this work. Several of the quadrupole based 2d ion traps are capable of mass selective isolation and activation of ions including ms3 analysis 7,911. Liquid chromatography mass spectrometry lcms thermo. Triple quadrupole linear ion trap mass spectrometer for the. Quadrupole ion trap an overview sciencedirect topics. Caprioli, vanderbilt university, nashville fast atom bombardment.
Yates, iii innovations in ion trap mass spectrometry have ex those of standard amino acid derivatives. Linear ion traps in mass spectrometry douglas 2005 mass. A mass spectrometer method and apparatus has a mass analyzer and a collision cell. Other types of mass spectrometers may also use a linear quadrupole. The quadrupole ion trap mass analyzer consists of three hyperbolic. Development of a quadrupole ion trap mass spectrometer for. Metzger, university of oldenburg electron ionization and fragmentation of organic ions and interpretation of ei mass spectra, j. Figure 1 shows its first mention in publication in 1953. Liquid chromatographymass spectrometry of domoic acid and. Thus, we use the first to obtain peptide maps with high mass accuracy, and subsequently obtain product ion spectra on any or all of the individual peptide ions observed in the peptide map.
A quadrupole ion trap ory of ion trap operation and give examples of the pracis a mass analyzer roughly the size of a tennis ball tical application of ion trap mass spectrometry to. As the name implies, it consists of four cylindrical rods, set parallel to each other. They are also called radio frequency rf traps or paul traps. Triple quadrupole linear ion trap mass spectrometer for. That the basic theory of operation of quadrupole devices was enunciated almost 100 years before the quadrupole ion trap and the related quadrupole mass. Triple quadrupole linear ion trap mass spectrometer for the analysis. Among the various types of mass analyzers, the quadrupole ion trap is probably best suited to miniaturization. Revised to reflect scientific and technological advances and new applications in the field, the second edition includes new chapters covering. Us6504148b1 quadrupole mass spectrometer with ion traps. Mass selective ion accumulation and fragmentation in a linear octopole ion trap external to a fourier transform ion cyclotron resonance mass spectrometer. Is independent of mz range, but depends on u, v, and.
Mass measurement and topdown hplcms analysis of intact. Ion trap lcms for general purpose mass spectrometers, ion trap mass spectrometers are costeffective and can easily be operated by students learning the. This configuration gives the linear ion trap a dual functionalityit can be used as a quadrupole mass filter or an ion trap. Pdf a fastresponse method for determination of atmospheric. The linear form of the trap can be used as a selective mass filter, or as an actual trap by creating a potential well for the ions along the axis of the electrodes.
The quadrupole ion trap, often used in conjunction with a quadrupole mass filter, remained a laboratory curiosity until 1979 when, at the american society for mass spectrometry conference in seattle, george stafford, jr. With its concise descriptions of the theory of ion motion and the principles of operation, quadrupole ion trap mass spectrometry, second edition is ideal for new users of quadrupole devices, as well as for scientists, researchers, and graduate and postdoctoral students working in analytical laboratories. A three section linear or twodimensional 2d quadrupole ion trap as a high performance mass spectrometer is described. While the paul ion trap storage solution is a threedimensional device, the quadrupole filter with its four parallel rods is a special twodimensional solution in general ion trap theory4. For quadrupole scans q3 is operated in the rfdc mode whereas in the trap mode q3 is operated as an ion trap by applying an rf potential to the quadrupole. Fourier transform ion cyclotron resonance fticr mass. The same mass spectrometer can be used to perform quadrupole or trap scans and switching from one to the other mode takes only a few milliseconds. Modified quadrupole ion trap mass spectrometer for infrared.
Broadband nondestructive ion detection is achieved in a quadrupole ion trap mass spectrometer by impulsive excitation of a collection of trapped ions of different masses and recording of ion image currents induced on a small detector electrode embedded in but isolated from the adjacent end cap electrode. When acquiring mass spectra with unit resolution for ions originating from a continuous source that is, ions being presented to the mass spectrometer as a steady stream the duty cycle of a quadrupole mass spectrometer. A hybrid tandem mass spectrometer is constructed by interfacing a quadrupole mass filter q to a quadrupole ion trap mass spectrometer qitms and is. Recently, linear ion traps lits have been combined with quadrupole q, time offlight tof and. A twodimensional quadrupole ion trap mass spectrometer core. Matrixassisted laser desorption ionization quadrupole ion trap mass spectrometry of peptides. The quadrupole ion trap is composed of two end cap electrodes at the entrance and the exit of the trap and a ring electrode shaped like a donut in the middle. An assessment of the application of a quadrupole ion trap mass spectrometer to the measurement of stable isotope ratios within the rosetta spaceflight mission has been undertaken. The quadrupole ion trap is constructed of three electrodes that, when held at appropriate potentials, cause the formation of a trapping. Us6797950b2 twodimensional quadrupole ion trap operated as. Apr 26, 2002 sional 3d ion trap mass spectrometers 12. The quadrupole ion trap mass spectrometera small solution. March, in encyclopedia of spectroscopy and spectrometry second edition, 2010.
A quadrupole ion trap mass spectrometer coupled with a gas chromatograph was used to study the reactions of two coeluting polychlorinated biphenyl pcb compounds, congener 77 3,3,4,4. Linear ion traps are finding new applications in many areas of mass spectrometry. Estimation of effective ion temperatures in a quadrupole ion trap. March, an introduction to quadrupole ion trap mass spectrometry, journal of mass spectrometry 32, 3569 1997. The quadrupole ion trap qit in a commercial mass spectrometer is based on the solutions to the mathieu differential, which analytically calculate the masses. Electrospray ion sources are compatible with many types of mass analyzers.
The mass spectrometer can answer the questions what is in the sample qualitative structural. Quadrupole ion trap mass spectrometry accounts of chemical. Broadband fourier transform quadrupole ion trap mass. The linear ion trap uses a set of quadrupole rods to confine ions radially and a static electrical potential onend electrodes to confine the ions axially. Quadrupole ion trap mass spectrometry, second edition is ideal for new users of quadrupole devices, as well as for scientists, researchers, and graduate and postdoctoral students working in analytical laboratories. The progress made in the field of quadrupole ion trap mass spectrometry in recent years has been quite extraordinary. Quadrupoles are the most widely used mass analyzers because they can tolerate a wide mz range and are of relatively low cost. A linear ion trap uses a set of quadrupole rods coupled with electrodes on each end to facilitate the ion trapping. The following chapter is a brief discussion of how a quadrupole mass filter actually achieves mass separation. For this application, we will consider the quadrupole ion trap as a mass analyzer. Jonscher department of molecular biotechnology box 357730, university of washington, seattle, washington 981957730 john r. The quadrupole mass analyzer qms, also known as a transmission quadrupole mass spectrometer, quadrupole mass filter, or quadrupole mass spectrometer, is one type of mass analyzer used in mass spectrometry. In a quadrupole mass spectrometer the quadrupole is the mass analyzer the component of the. Mass spectrometry is an analytic method that employs ionization and mass analysis of compounds to determine the mass, formula and structure of the compound being analyzed.
As a starting point, ask yourself why the peak in an lc chromatogram is a peak, not a line. Move from compound discovery into targeted quantitation for your sample type by choosing from a broad portfolio of thermo scientific q exactive quadrupole orbitrap mass spectrometers. The quadrupole ion trap is constructed of three electrodes that, when held at appropriate potentials, cause the formation of a trapping pseudopotential. Mass analysis is performed by ejecting ions radically out a slot formed in one of the rods using the mass selective instability mode of operation. Resolution can be controlled by using appropriate end section potentials to control the axial spread of the ion cloud. The use of a linear or twodimensional 2d quadrupole ion trap as a high performance mass spectrometer is demonstrated. Veith, technical university of darmstadt field desorption, r. Quadrupole ion trap mass spectrometer qitms to allow for continuous cabin air major constituents analysis in addition to a voc measurement performed once a day. The two most common types of ion traps are the penning trap and the paul trap quadrupole ion trap 59. The acetyl cation has also been shown to react selectively with alkenes, via cycloaddition at the double bond. Us7034294b2 twodimensional quadrupole ion trap operated. A definitive reference, completely updated published in 1989, the first edition of this book, originally entitled quadrupole storage mass spectrometry, quickly became the definitive reference in analytical laboratories worldwide. When non mass spectrometrists are talking about mass spectrometry it rather often sounds as if they were telling a story out of poes tales of mystery and imagina tion.
A mass spectrometer is an analytical instrument that can separate charged molecules according to their mass tocharge ratio. Structural characterization of cardiolipin by tandem. Quadrupole ion trap mass spectrometry, 2nd edition. Range of stability for qms trajectories obeying mathieus equation. Many mass spectrometry based experiments, not just ion traps, have since exploited the use of coherent laser light sources for. When vme is introduced into the quadrupole ion trap, the most abundant ions present are the acetyl cation mz 43 and the vme molecular ion mz 58. Here, we evaluate the sensitivity and quantification accuracy of rts ms3 on the orbitrap eclipse mass spectrometer for. Resonance ejection and excitation are utilized to enhance mass analysis and to allow isolation and activation of ions for ms n capability. A microscale planar linear ion trap mass spectrometer.
These instruments contain four cylindrical rods placed in parallel in a radial. Lcms methodology for the analysis of domoic acid and lipophilic toxins in shellfish was developed using a hybrid triple quadrupole linear ion trap mass spectrometer. In theory this works but in practice we do something. The slot geometry is optimized to enable ions of different mass ranges to be scanned out of differently dimensioned slots. New quadrupoleion traporbitrap mass spectrometer combined.
International journal of mass spectrometry 2000, 198 12, 1120. The slot geometry is optimized to yield high ejection efficiencies. Us20030183759a1 twodimensional quadrupole ion trap. Quadrupole ion trap mass spectrometry wiley online books. A novel highcapacity ion trapquadrupole tandem mass. It is used as a component of a mass spectrometer or a trapped ion quantum computer. In a linear ion trap, ions are confined radially by a two. An introduction to quadrupole ion trap mass spectrometry. Common mass analyzers include quadrupole, ion trap, fourier transform ion cyclotron resonance fticr, and timeofflight tof instruments skoog and leary, 1992a,b,c,d.
Jan 27, 2005 with its concise descriptions of the theory of ion motion and the principles of operation, quadrupole ion trap mass spectrometry, second edition is ideal for new users of quadrupole devices, as well as for scientists, researchers, and graduate and postdoctoral students working in analytical laboratories. Critical experimental parameters for infrared multiple photon dissociation irmpd on this instrument are characterized. Hcd ms 2 and ms 3 spectra of permethylated chicken ovalbumin glycans. Wong bioanalytical systems west lafayette, in 4790682 r. Pdf matrixassisted laser desorption ionizationquadrupole. Mattauch and herzog in 1934 and recently revised into a new type of mass filter, time of flight ms by stephens in 1946, ion cyclotron resonance ms by hipple and thomas in 1949, quadrupole ms by steinwedel in 1953, and ion trap ms by paul and dehmelt in the 1960s.
March and others published quadrupole ion trap mass spectrometry find, read and cite all the research you need on researchgate. Graham cooks department of chemistry purdue university west lafayette, in 47907 corresponding author email. In this study, we have developed a novel membrane sampling cryogenic focusing system for the determination of isoprene in a quadrupole ion trap utilizing vinyl methyl ether vme as a ci reagent coupled with cid. Bioanalytical lcms 22 22 the mass peak in the quadrupole mass spectrum a common phrase in texts on ms says. Mass resolution study in the cylindrical ion trap by using. What is a mass spectrometer and what is mass spectrometry. Qitms or paul traps were invented 6 decades ago 7, but their potential use as a mass spectrometer for noble gases was explored only recently.
Quadrupole ion trap mass spectrometry, 2nd edition mass. The fragment ions are then scanned out axially by application of suitable excitation to the ions. Orbitrap eclipse quadrupoleion trap orbitrap instrument, building on the sps ms3 method used with previous generations of this platform. Experimental sectionall experiments were carried out using a varian saturn 2000 gas chromatograph ion trap mass spectrometer. The image currents are directly measured using a simple differential preamplifier, filter.
Ion chromatography is ideal for mass spectrometry ms analytes are provided already in the ionic form needed for ms detection thermo scientific dionex suppressorsallow the use of standard ic eluents and methods and improve ms sensitivity, signal stability and saltmatrix tolerance icms may use existing methods from standalone ic. The earliest application of 2d multipole ion traps in mass spectrometry involved the use of the collision cell of a triple quadrupole instru ment for. Wesener, bayer industry services, leverkusen chemical ionization, j. Quadrupole ion trap mass spectrometer for ice giant. The search for a theory of ion motion is not a long one since it has been found that the motion of ions in a quadrupole eld can be described mathematically by the. The quadrupole ion trap mass spectrometer a small solution to a big challenge karen r. A twodimensional quadrupole ion trap mass spectrometer. An introduction to quadrupole ion trap mass spectrometry pdf.
Learn how an ion trap works, compare linear ion trap mass spectrometers from the worlds leading provider, and better understand why these affordable, flexible instruments are a top choice for labs looking for fullscan sensitivity, indepth analysis, and an ion trap lcms solution with automation so all users, despite experience, will have more confidence in their results. Journal of the american society for mass spectrometry 2007, 18 3, 466478. The quadrupole ion trap is composed of two end cap electrodes at the entrance and the exit of the trap and. An orbitrap mass spectrometer consists of an inner spindlelike electrode and an outer barrellike electrode. Quadrupole and ion trap mass analyzers rev20040526. Upon exiting the drift tube, ions enter a quadrupole mass filter where a specific mass tocharge mz ratio can be selected prior to collisional activation in an octopole. Quadrupole ion trap mass spectrometer update based on the. Principles of quadrupole and ion trap mass analyzers. A view at the turn of the century a personal account of the quadrupole ion trap researches carried out in my laboratory and in collaboration. The majority of mass spectrometers installed and used are based on quadrupole mass analyzer technology. Mass spectrometry was coupled with icp as a means of sample introduction in 1980. Modifications to a paultype quadrupole ion trap mass spectrometer providing optical access to the trapped ion cloud as well as hardware and software for coupling to a tabletop ir optical parametric oscillator laser opo are detailed.
The ion trap is an attractive choice due to its mechanical simplicity and compact nature, and potentially offers versatile performance through its facile control via software. The qitms quadrupole ion trap mass spectrometer is an extraordinary instrument in that it is physically small the entire electrode assembly can be held in the palm of ones hand compared with magnetic and electric sector instruments, it is relatively inexpensive, it is one of the most, if not the most, sensitive mass spectrometers and, since several mass. The orbitrap, introduced in 2005, is based on the kingdon trap 4. B representation of an improved scheme for linkedscan analysis using a tandem mass spectrometer consisting of an ion trap followed by a collision cell followed by a mass analyzer. Mass analyzers types of mass spectrometer magnetic sector quadrupole ion trap timeofflight hybrid sector trap, quadtof etc mass spectrometers separate ions according to their mass tocharge mz ratios. Comparison of an iontrap and a quadrupole mass spectrometer. Mass spectrometry ms is widely recognized as a powerful analytical tool for molecular re search. Recent stateoftheart upgrades to vcam are currently undergoing technological demonstration onboard the international space station as the spacecraft atmosphere monitor madzunkov. Increasing the upper masscharge limit of a quadrupole ion trap. Uv photodissociation action spectroscopy of haloanilinium. For routine quantitation a scheduled selected reaction monitoring srm method for the analysis of domoic acid, okadaic acid, dinophysistoxins, azaspiracids, pectenotoxins, yessotoxins, gymnodimines, spirolides, and pinnatoxins. Determination of vanillin, ethyl vanillin, and coumarin in. These instruments contain four cylindrical rods placed in parallel in a radial array. Mixture analysis using a quadrupole mass filterquadrupole ion.
Fundamentals of ion trap mass spectrometry presents an account of the development and theory of the quadrupole ion trap and its utilization. Quadrupole ion traps qits are versatile platforms for performing. Us6504148b1 quadrupole mass spectrometer with ion traps to. Mathematical analysis of the quadrupole mass filter the x and ymotions of an ion with mass m and charge q are described by the following equations of motion for the three separate coordinate axes. However, qit designs with theoretical configuration have problematic mass shifts, which give rise to errors in the mass analysis of specific compounds. Basics of mass spectrometry in the clinical laboratory. Ionization techniques mass analyzers single quadrupole, triple quadrupole, ion trap sim, srm, ion ratios and product ion spectra for confirmation high resolution mass analyzers and data acquisition nominal mass vs exact mass comparison of mass analyzers. Glycan structural elucidation on a novel quadrupole dualcell. An ion trap mass spectrometer may incorporate a penning trap 1, paul trap 2 or the kingdon trap 3.
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